| 货号: S221194 |
| 产品全名: COX4I1 兔多抗 |
| 基因符号 COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1 |
| UNIPROT ID: P13073 (Gene Accession – NP_001852 ) |
| 背景: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3′ of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| 抗原: Synthetic peptide of human COX4I1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer tissue using 221194(COX4I1 Antibody) at a dilution of 1/25(Cytoplasm). | 
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 221194(Anti-COX4I1 Antibody) at a dilution of 1/25. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: A549, PC3, 293T, NIH/3T3, Hela, HepG2, Mouse skeletal muscle tissue lysates;
Primary antibody: 221194(COX4I1 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds | |
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