武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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AP2A1 Rabbit pAb

Anti-AP2A1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S221165

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S221165
产品全名:   AP2A1 兔多抗
基因符号   ADTAA; CLAPA1; AP2-ALPHA
UNIPROT ID:   O95782 (Gene Accession – NP_055018 )
背景:   This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.
抗原:   Synthetic peptide of human AP2A1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221165(AP2A1 Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221165(Anti-AP2A1 Antibody) at dilution 1/55.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: NIH/3T3 and Hela cell lysates;
Primary antibody: 221165(AP2A1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
           引用文献