| 货号: S220790 |
| 产品全名: PDE4A 兔多抗 |
| 基因符号 PDE4; DPDE2; PDE46 |
| UNIPROT ID: P27815 (Gene Accession – NP_001104777 ) |
| 背景: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| 抗原: Synthetic peptide of human PDE4A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220790(PDE4A Antibody) at a dilution of 1/40(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220790(Anti-PDE4A Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220790(Anti-PDE4A Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D262029(Anti-PDE4A Antibody) at dilution 1/40. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, A172 cells;
Primary antibody: 220790(PDE4A Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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