| 货号: S220748 |
| 产品全名: RHEB 兔多抗 |
| 基因符号 RHEB2 |
| UNIPROT ID: Q15382 (Gene Accession – NP_005605 ) |
| 背景: This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. |
| 抗原: Synthetic peptide of human RHEB |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220748(RHEB Antibody) at a dilution of 1/40(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220748(Anti-RHEB Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 220748(Anti-RHEB Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D261955(Anti-RHEB Antibody) at dilution 1/40. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Human brain malignant glioma tissue, Jurkat cells, Raji cells, 231 cells, human fetal brain tissue, Hela cells;
Primary antibody: 220748(RHEB Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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