| 货号: S219982 |
| 产品全名: RNF126 兔多抗 |
| 基因符号 |
| UNIPROT ID: Q9BV68 (Gene Accession – NP_919442 ) |
| 背景: The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. RNF126 interacts with p21 and RNF126 over-expression increased p21 protein ubiquitination in an E3 ligase activity-dependent manner. RNF126 knockdown induced loss of cell viability in MDA-MB-231 and PC-3 can be partially rescued by depletion of p21. RNF126 stable knockdown in PC3 inhibited tumor growth in SCID mice. |
| 抗原: Synthetic peptide of human RNF126 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219982(RNF126 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 219982(Anti-RNF126 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 219982(Anti-RNF126 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D260729(Anti-RNF126 Antibody) at dilution 1/20. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat cells, K562 cells;
Primary antibody: 219982(RNF126 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
|
