| 货号: S219690 |
| 产品全名: CDC6 兔多抗 |
| 基因符号 CDC18L; HsCDC6; MGORS5; HsCDC18 |
| UNIPROT ID: Q99741 (Gene Accession – NP_001245.1 ) |
| 背景: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. |
| 抗原: Synthetic peptide of human CDC6 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 219690(CDC6 Antibody) at a dilution of 1/25(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 219690(Anti-CDC6 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 219690(Anti-CDC6 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D260157(Anti-CDC6 Antibody) at dilution 1/25. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: K562 cells;
Primary antibody: 219690(CDC6 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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