武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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ARF6 Rabbit pAb

Anti-ARF6 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S219637

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S219637
产品全名:   ARF6 兔多抗
基因符号   
UNIPROT ID:   P62330 (Gene Accession – NP_001654 )
背景:   This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
抗原:   Synthetic peptide of human ARF6
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: Oct-50;WB: 500-2000;ELISA: 2000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 219637(ARF6 Antibody) at a dilution of 1/15(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 219637(Anti-ARF6 Antibody) at dilution 1/15.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 cells, 231 cells;
Primary antibody: 219637(ARF6 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
           引用文献