| 货号: S217347 |
| 产品全名: DCTN2 兔多抗 |
| 基因符号 RBP50; DCTN50; HEL-S-77; DYNAMITIN |
| UNIPROT ID: Q13561 (Gene Accession – BC000718 ) |
| 背景: This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. |
| 抗原: Fusion protein of human DCTN2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217347(DCTN2 Antibody) at a dilution of 1/25(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217347(Anti-DCTN2 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217347(Anti-DCTN2 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D222213(Anti-DCTN2 Antibody) at dilution 1/25. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T cells, mouse brain tissue, A375 cells;
Primary antibody: 217347(DCTN2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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