| 货号: S217269 |
| 产品全名: SEPTIN7 兔多抗 |
| 基因符号 CDC3; CDC10; SEPT7; SEPT7A; NBLA02942 |
| UNIPROT ID: Q16181 (Gene Accession – BC067264 ) |
| 背景: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011] |
| 抗原: Fusion protein of human SEPTIN7 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217269(SEPTIN7 Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217269(Anti-SEPTIN7 Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217269(Anti-SEPTIN7 Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222079(Anti-SEPTIN7 Antibody) at dilution 1/60. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cells;
Primary antibody: 217269(SEPTIN7 Antibody) at dilution 1/430;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds | |
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