| 货号: S217184 |
| 产品全名: ALPP 兔多抗 |
| 基因符号 ALP; PALP; PLAP; PLAP-1 |
| UNIPROT ID: P05187 (Gene Accession – BC009647 ) |
| 背景: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3′ untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. |
| 抗原: Fusion protein of human ALPP |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217184(ALPP Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217184(Anti-ALPP Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217184(Anti-ALPP Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D221938(Anti-ALPP Antibody) at dilution 1/60. | 
Gel: 15%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 cells, human placenta tissue;
Primary antibody: 217184(ALPP Antibody) at dilution 1/1550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second | |
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