| 货号: S216936 |
| 产品全名: ADAM32 兔多抗 |
| 基因符号 |
| UNIPROT ID: Q8TC27 (Gene Accession – BC026169 ) |
| 背景: A number of a disintegrin and metalloprotease (ADAM) family members are expressed in mammalian male reproductive organs such as testis and epididymis. The cDNA sequence of ADAM32 has an open reading frame which encodes 750 (mouse ADAM32) and 787 (human ADAM32) amino acids, sharing 69% homology between the two species. Comparison of amino acid sequences showed that ADAM32 is highly homologous to ADAM2 and ADAM3, sperm surface proteins required for fertilization. We found that the ADAM32 gene is expressed predominantly in the testis. |
| 抗原: Fusion protein of human ADAM32 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 15-50;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216936(ADAM32 Antibody) at a dilution of 1/15(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216936(Anti-ADAM32 Antibody) at dilution 1/15. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 216936(Anti-ADAM32 Antibody) at a dilution of 1/15. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D221499(Anti-ADAM32 Antibody) at dilution 1/15. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human testis tissue;
Primary antibody: 216936(ADAM32 Antibody) at dilution 1/100;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes | |
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