武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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NME1 Rabbit pAb

Anti-NME1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216682

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216682
产品全名:   NME1 兔多抗
基因符号   NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1
UNIPROT ID:   P15531 (Gene Accession – BC000293 )
背景:   This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ‘A’ (encoded by this gene) and ‘B’ (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
抗原:   Fusion protein of human NME1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 216682(NME1 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 216682(Anti-NME1 Antibody) at dilution 1/40.

Gel: 10+12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 231 cells, A549 cells, human liver cancer tissue;
Primary antibody: 216682(NME1 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
           引用文献