| 货号: S216638 |
| 产品全名: MCM5 兔多抗 |
| 基因符号 CDC46; P1-CDC46 |
| UNIPROT ID: P33992 (Gene Accession – BC003656 ) |
| 背景: The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. |
| 抗原: Fusion protein of human MCM5 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216638(MCM5 Antibody) at a dilution of 1/40(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216638(Anti-MCM5 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 216638(Anti-MCM5 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D220960(Anti-MCM5 Antibody) at dilution 1/40. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HepG2 cells, K562 cells, Jurkat cells, 231 cells, hela cells;
Primary antibody: 216638(MCM5 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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