| 货号: S216384 |
| 产品全名: CAPN7 兔多抗 |
| 基因符号 PALBH; CALPAIN7 |
| UNIPROT ID: Q9Y6W3 (Gene Accession – BC056202 ) |
| 背景: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The function of the protein encoded by this gene is not known. An orthologue has been found in mouse but it seems to diverge from other family members. The mouse orthologue is thought to be calcium independent with protease activity. |
| 抗原: Fusion protein of human CAPN7 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216384(CAPN7 Antibody) at a dilution of 1/20(Cytoplasm and Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216384(Anti-CAPN7 Antibody) at dilution 1/20. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Human normal liver tissue, mouse liver tissue, mouse intestines tissue, Mouse pancreas tissue, hepg2 cells, HT29 cells, mouse heart tissue;
Primary antibody: 216384(CAPN7 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
|
