| 货号: S216380 |
| 产品全名: CAPN1 兔多抗 |
| 基因符号 CANP; muCL; CANP1; SPG76; CANPL1; muCANP |
| UNIPROT ID: P07384 (Gene Accession – BC017200 ) |
| 背景: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. |
| 抗原: Fusion protein of human CAPN1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216380(CAPN1 Antibody) at a dilution of 1/75(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216380(Anti-CAPN1 Antibody) at dilution 1/75. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 216380(Anti-CAPN1 Antibody) at a dilution of 1/75. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D220328(Anti-CAPN1 Antibody) at dilution 1/75. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562, Jurkat and Hela cell lysates;
Primary antibody: 216380(CAPN1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds | |
|
