武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PKM Rabbit pAb

Anti-PKM Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216217

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216217
产品全名:   PKM 兔多抗
基因符号   PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
UNIPROT ID:   P14618 (Gene Accession – BC007640 )
背景:   This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
抗原:   Fusion protein of human PKM
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 500-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Epigenetics and Nuclear Signaling, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216217(PKM Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216217(Anti-PKM Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 30 μg;
Lane: Hela cells;
Primary antibody: 216217(PKM Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
           引用文献