| 货号: S213940 |
| 产品全名: PPARD 兔多抗 |
| 基因符号 FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB |
| UNIPROT ID: Q03181 (Gene Accession – NP_803184 ) |
| 背景: This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants. |
| 抗原: Synthetic peptide of human PPARD |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Metabolism, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 213940(PPARD Antibody) at a dilution of 1/55(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 213940(Anti-PPARD Antibody) at dilution 1/55. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 213940(Anti-PPARD Antibody) at a dilution of 1/55. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D161088(Anti-PPARD Antibody) at dilution 1/55. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HEPG2 cell lysate;
Primary antibody: 213940(PPARD Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds | |
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