| 货号: S211543 |
| 产品全名: SEPTIN2 兔多抗 |
| 基因符号 DIFF6; NEDD5; SEPT2; NEDD-5; Pnutl3; hNedd5 |
| UNIPROT ID: Q15019 (Gene Accession – BC033559 ) |
| 背景: Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri. |
| 抗原: Fusion protein of human SEPTIN2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 211543(SEPTIN2 Antibody) at a dilution of 1/30(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 211543(Anti-SEPTIN2 Antibody) at dilution 1/30. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cells;
Primary antibody: 211543(SEPTIN2 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10S | |
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