武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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TRIM27 Rabbit pAb

Anti-TRIM27 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217904

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217904
产品全名:   TRIM27 兔多抗
基因符号   RFP; RNF76
UNIPROT ID:   P14373 (Gene Accession – BC013580 )
背景:   This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.
抗原:   Fusion protein of human TRIM27
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 45-300;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 217904(TRIM27 Antibody) at a dilution of 1/65(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 217904(Anti-TRIM27 Antibody) at dilution 1/65.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217904(Anti-TRIM27 Antibody) at a dilution of 1/65.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D223342(Anti-TRIM27 Antibody) at dilution 1/65.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human testis tissue, 293T and A172 cell lysates;
Primary antibody: 217904(TRIM27 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
           引用文献