武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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SPHK2 Rabbit pAb

Anti-SPHK2 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217166

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217166
产品全名:   SPHK2 兔多抗
基因符号   SK 2; SK-2; SPK 2; SPK-2
UNIPROT ID:   Q9NRA0 (Gene Accession – BC006161 )
背景:   This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
抗原:   Fusion protein of human SPHK2
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Metabolism
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217166(SPHK2 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217166(Anti-SPHK2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 217166(Anti-SPHK2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D221906(Anti-SPHK2 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: RAMOS cell lysate;
Primary antibody: 217166(SPHK2 Antibody) at dilution 1/300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献