武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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SIAH1 Rabbit pAb

Anti-SIAH1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217815

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217815
产品全名:   SIAH1 兔多抗
基因符号   SIAH1A
UNIPROT ID:   Q8IUQ4 (Gene Accession – BC035562 )
背景:   This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson’s disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
抗原:   Fusion protein of human SIAH1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 200-400;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cell Biology, Neuroscience
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 217815(SIAH1 Antibody) at a dilution of 1/200(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 217815(Anti-SIAH1 Antibody) at dilution 1/200.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: LO2 cell, Mouse liver tissue, Mouse kidney tissue lysates;
Primary antibody: 217815(SIAH1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 70 seconds
           引用文献