武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PLS3 Rabbit pAb

Anti-PLS3 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217697

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217697
产品全名:   PLS3 兔多抗
基因符号   BMND18; T-plastin
UNIPROT ID:   P13797 (Gene Accession – BC039049 )
背景:   Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.
抗原:   Fusion protein of human PLS3
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217697(PLS3 Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217697(Anti-PLS3 Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 217697(Anti-PLS3 Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D222885(Anti-PLS3 Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T and BGC-823 cell lysates;
Primary antibody: 217697(PLS3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
           引用文献