武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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MCM3AP Rabbit pAb

Anti-MCM3AP Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217467

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217467
产品全名:   MCM3AP 兔多抗
基因符号   GANP; SAC3; MAP80
UNIPROT ID:   O60318 (Gene Accession – BC104958 )
背景:   The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability.
抗原:   Fusion protein of human MCM3AP
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217467(MCM3AP Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217467(Anti-MCM3AP Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217467(Anti-MCM3AP Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222405(Anti-MCM3AP Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, 293T cells;
Primary antibody: 217467(MCM3AP Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
           引用文献