武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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IBSP Rabbit pAb

Anti-IBSP Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S220407

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S220407
产品全名:   IBSP 兔多抗
基因符号   BSP; BNSP; SP-II; BSP-II
UNIPROT ID:   P21815 (Gene Accession – NP_004958 )
背景:   The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
抗原:   Synthetic peptide of human IBSP
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Stem Cells
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220407(IBSP Antibody) at a dilution of 1/50(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220407(Anti-IBSP Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: K562 cell lysate;
Primary antibody: 220407(IBSP Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
           引用文献