武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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DCTN1 Rabbit pAb

Anti-DCTN1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217346

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217346
产品全名:   DCTN1 兔多抗
基因符号   P135; DP-150; DAP-150
UNIPROT ID:   Q14203 (Gene Accession – BC006163 )
背景:   This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
抗原:   Fusion protein of human DCTN1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 100-300;WB: 200-1000;ELISA: 2000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Neuroscience
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217346(DCTN1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217346(Anti-DCTN1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217346(Anti-DCTN1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D222212(Anti-DCTN1 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 217346(DCTN1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
           引用文献