武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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CD27 Rabbit pAb

Anti-CD27 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217262

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217262
产品全名:   CD27 兔多抗
基因符号   T14; S152; Tp55; TNFRSF7; S152. LPFS2
UNIPROT ID:   P26842 (Gene Accession – BC012160 )
背景:   The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
抗原:   Fusion protein of human CD27
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cancer, Immunology, Stem Cells
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217262(CD27 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217262(Anti-CD27 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217262(Anti-CD27 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222070(Anti-CD27 Antibody) at dilution 1/60.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Raji cells, PC3 cells, lovo cells;
Primary antibody: 217262(CD27 Antibody) at dilution 1/727;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
           引用文献