武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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BCAT1 Rabbit pAb

Anti-BCAT1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217202

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217202
产品全名:   BCAT1 兔多抗
基因符号   BCT1; PP18; BCATC; ECA39; MECA39; PNAS121
UNIPROT ID:   P54687 (Gene Accession – BC033864 )
背景:   This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.
抗原:   Fusion protein of human BCAT1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217202(BCAT1 Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217202(Anti-BCAT1 Antibody) at dilution 1/55.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217202(Anti-BCAT1 Antibody) at a dilution of 1/55.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D221976(Anti-BCAT1 Antibody) at dilution 1/55.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Ramos cell lysate;
Primary antibody: 217202(BCAT1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献